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![]() Unless you are doing massive amounts of sequence alignment, the clustalW2 webserver is quicker, more sensitive and way easier to use. Use Bio::Tools::Run::Alignment::Clustalw What do the dots and dashes mean (Entry 1 of 2) 1 : formed of or as if of alternating dots and dashes run a dot-and-dash line across the sheet. ![]() Include a definition of an environmental variable CLUSTALDIR inĮvery script that will use this Clustalw wrapper module, e.g.:īEGIN Setenv CLUSTALDIR /home/username/clustalw1.8 Returns a clustalw executable on your system.ĭefine an environmental variable CLUSTALDIR which is aĭirectory which contains the 'clustalw' application:Įxport CLUSTALDIR=/home/username/clustalw1.8 Putting spaces before and after an em dash is a matter of preference just. This message means 'S O S' (S '.' and O is '-'), the distress signal. Use one before the parenthetical information and one after it. For this usage, make sure you use two em dashes. Using em dashes instead of parentheses puts the focus on the information between the em dashes. Make sure the clustalw executable is in your path so that Em dashes are often used to set off parenthetical information. From the documentation here are common problems: What you have appears to be the alignment of a nucleotide sequence that has gone horribly, horribly wrong.Īs a first guess without any more details I would say it doesn't look like you have installed clustalw correctly or pointed your bioperl to it. You are right in that this isn't a proper alignment, however I'm not sure it is a bug. Dots are used to show specifically point mutations where the biochemical properties are mostly conserved, but the residue has changed.įrom original question, not relevant to question as it stands. Head2 -UGGAGAAU.-.-gcgggcaucgaucccgcUUCCUCUCGCAUGCUAA.Īs WYSIWYG touches on in the comments, usually dashes mean that there are gaps in the alignment as the result of an indel event. Head1 -UGGAGAAU.G-.-ugggcguc.gaucccacUUCCUCUCGCAUGCUAA. Head2 -UGGAGAAU.-.-gcgggcaucgaucccgcUUCCUCUCGCAUGCUAA.GCGAGCG.-.-cucuaccauu. Head1 -UGGAGAAU.G-.-gcucuaccacuugagcuaauucccc. These are RNA sequences, including pseudogenes, so there are lots of palindromic sequences. I was unable to find the sequences I used in the original message but these two pose a similar pattern. What is the meaning of dots and dashes? Is this natural in a clustalw output? Am I getting a bug? I am also using my $new=$aln->remove_columns(,'.') Īnd got an output like head1 -.GG-.-gggaguggugugguuacgaaugUGGCCUCUGC-A Anonymous 1y ago LIVE Points 59 Rating In Morse code, dot dot dash is the letter 'U'. I am converting outputs in stockholm format to clustalw using Bio::AlignIO->new(-file => "$ARGV", -format => "stockholm") andīio::AlignIO ->new (-format =>'clustalw') ![]() While you are getting the Businesses built to produce those quantities, i.e. ![]() several thousand per month, or enough to saturate the Viceroy's demands which is about a total of 3500 per month or more. ![]() That should solve most problems with the Auto Trade Routes.īut because of the 1000 Gold per Day COST of that Warehouse, you should only do this after you are able to sell a lot of colonial commodities regularly to Maracaibo, i.e. When you are Unloading to the Warehouse I think it is possible to tell the Trade Route to not pay any attention to demand and just unload it. Then when the prices are right you can have the Warehouse sell them to the town market. One idea that might work for Auto Trade Routes would be to build a Warehouse at Maracaibo, and then have all colonial commodities UNLOADED to the WAREHOUSE. In order to get the best prices for colonial commodities at Maracaibo it is best to manually control the convoys. I always used manual control and avoided having to deal with that problem. Because large amounts are constantly being sold to it and then being picked by the Treasure Fleets, the demands and stockpiles are constantly changing, which messes up making the Trade Routes work consistently. Many players have trouble trying to use Trade Routes to deliver colonial commodities to Maracaibo. If you need help on warehouse settings, I just published a guide about them I'd like to see something implemented where you can just go by price and not demand, like how the warehouse works with a "sell until price" setting If the priority is set evenly for all of the ports, it will save some of the commodities to sell to other ports that are demanding the commodity. The convoy will assess the demand of the good for all ports on the route. The trade routes are interesting in the way they work. Additionally, you can set the warehouse to buy that good from the town.Įdit: That town with the 4 red, on the trade route set up you can increase that town's priority for that good. On one of the warehouse tabs, you can click checkboxes next to all the goods you want to trade or sell to the town. I find the traderoutes very buggy, often find my ship getting full and checking the town it last went to and being 4 red on items the ship has lots of. Now what I do is I have the convoys load it all into a warehouse in the capital, and have the warehouse sell to the city based on the priceĬan you actually auto-sell from the warehouse? Last stop is Maracaibo, and I have it set to SELL, 5 star priority, quantity at 5 squares, and price is Whatever.ĭoing this I oversell, but since it was my first game and I was just learning it didn't matter. Originally posted by Rabid Puma:I set my trade route for commodities thus:Įverything OFF in Seville except Cotton. Auto Trade Routes should come after you get the colonial commodity productions established. Forget Auto Trade Routes at the start of the Game, start buying whatever colonial commodities that are being produced by AI owned businesses in nearby towns, and then start building your own Businesses with the profits. In order to finish the Spanish Campaign you are going to have to build and own even more businesses than that, so why wait?īUILD 4 Cotton and 4 Tobacco Businesses in Seville, and 4 Coffee and 4 Cacao Businesses in Puerto Cabezas. You have Tasks that will eventually REQUIRE you to own those 4 Businesses in each colonial commodity, so do it quickly (instead of waiting) at the start of the Spanish Campaign Game, and start getting the Experience Points and cash profits as soon as possible. I was generating a Fame Point (in the Spanish Campaign in the Beta) about every 2 months by doing random tasks as well as the colonial commodity deliveries. You "just" need 4 owned businesses of each colonial commodity (cotton, tobacco, coffee, and cacao), and then start delivering the production to Maracaibo. Since you need a lot of Fame Points, make a point of maxing out the demand for colonial commodities by the Viceroy EVERY month. I sold 3500 to 4000 total units of colonial goods to Maracaibo EVERY Month for over a game year (in the Beta) and made a good profit doing so ( profit of 150k to 200k per month), by using manual control of the convoys delivering the colonial commodities, AND I got 100 Experience Points (towards Fame Points) every month from the Viceroy. To get the best prices at Maracaibo for colonial commodities, including Coffee and Cacao, you want to use manual control of the convoys so that they wait to sell when the prices are good (high), which happens after the 2 Spanish Treasure Fleets leave (they always arrive around the 8th day of the month). Take the Coffee and Cacao directly to Maracaibo, Seville is a LOT farther away from Maracaibo than Puerto Cabezas, just have the ships going from Seville to Maracaibo stop at Puerto Cabezas on the way to Maracaibo. ![]() The poem begins with what sounds like a march when read aloud, echoing the boost the Calvary may have been given at the time of the battle. An example of this can be easily seen (stressed syllables bolded) in the line "’Forward the Light Brigade!’" The poem’s meter is consistent throughout. Lines present a meter in which two unstressed syllables follow a stressed syllable. Though there are rhymed lines in the poem, it does not follow a consistent rhyme scheme. The poem is composed of six stanzas of varying length. The poem was written as a commemoration of a specific event in the 1854 Battle of Balaclava. JSTOR 40371812.Alfred, Lord Tennyson wrote " The Charge of the Light Brigade" in 1854. " 'The Charge of the Light Brigade': The Creation of a Poem". Shannon, Edgar & Ricks, Christopher (1985).Archived from the original on 26 August 2014. : CS1 maint: location missing publisher ( link) Hell riders : the true story of the charge of the Light Brigade. Hallam Lord Tennyson and annotated by Alfred Lord Tennyson (London: Macmillan, 1908), II, 369 Shannon and Ricks 8. Its purpose was to shame the British public into offering financial assistance. His poem focuses on the terrible hardships faced in old age by veterans of the Crimean War, as exemplified by the cavalry men of the Light Brigade. Rudyard Kipling wrote " The Last of the Light Brigade" (1891) some 40 years after the appearance of "The Charge of the Light Brigade". Tennyson recited this poem onto a wax cylinder in 1890. For this he rethought the revisions in Maud and Other Poems, and this rethought version was used for the second edition of Maud, in 1856. Īt the suggestion of Jane, Lady Franklin, Tennyson sent a thousand copies of a single-sheet version of the poem to be distributed among soldiers in the Crimea. These changes were criticized by several, including both Tennyson and Tuckerman. Tennyson made revisions to the poem due to criticisms by the American poet Frederick Goddard Tuckerman and others these were published in Tennyson's volume Maud and Other Poems (1855). Tennyson wrote the poem based on two articles published in The Times: the first, published on 13 November 1854, contained the sentence "The British soldier will do his duty, even to certain death, and is not paralyzed by the feeling that he is the victim of some hideous blunder," the last three words of which provided the inspiration for his phrase "Some one had blunder'd." The poem was written in a few minutes on 2 December of the same year, based on a recollection of The Times's account Tennyson wrote other similar poems, like "Riflemen Form!", in a very similar manner. The poem was written after the Light Cavalry Brigade suffered great casualties in the Battle of Balaclava. Scholars speculate that Tennyson created his pen names because these verses used a traditional structure Tennyson employed in his earlier career but suppressed during the 1840s, worrying that poems like "The Charge of the Light Brigade" (which he initially signed only A.T.) "might prove not to be decorous for a poet laureate". History Composition Tennyson as photographed by Lewis Carroll in 1857ĭuring 1854, when the United Kingdom was engaged in the Crimean War, Tennyson wrote several patriotic poems under various pseudonyms. The poem was subsequently revised and expanded for inclusion in Maud and Other Poems (1855). He was the Poet Laureate of the United Kingdom at the time. He wrote the original version on 2 December 1854, and it was published on 9 December 1854 in The Examiner. " The Charge of the Light Brigade" is an 1854 narrative poem by Alfred, Lord Tennyson about the Charge of the Light Brigade at the Battle of Balaclava during the Crimean War. The Charge of the Light Brigade (Tennyson) at Wikisource Richard Caton Woodville Jr.'s 1894 painting of the eponymous Charge of the Light Brigade, the event that inspired the poem ![]() Entdecken sie 'precious' im wrterbuch Collins COBUILD Advanced Learner’s Dictionary. These examples are programmatically compiled from various online sources to illustrate current usage of the word 'precious.' Any opinions expressed in the examples do not represent those of Merriam-Webster or its editors. Adjektiv If you say that something such as a resource is precious, you mean that it is valuable and should not be wasted or used badly. 2023 Tell me about your innovative ways of cutting and presenting precious stones, such as free-form cutting or slicing them to expose their raw, natural beauty. The meaning of PRECIOUS is of great value or high price. ![]() under strict post-pandemic rules to balance the interests of those enjoying the scene - and those finding it a nuisance. 2023 But time was precious the entire inviting setup would have to be dismantled by 10 p.m. Gamble deploys a broadly phenomenological definition of culture: emphasis. 2023 His enduring vision is to leave a precious legacy of education, aiming to transform QIU into a bustling eco-friendly city. in Sussex represent precious moments in time: snapshots of ancient events. To live happily and enjoy every moments which is precious as your question even shows that every moments of our. 0:00 / 6:24 Adorable Baby Sleeping With Daddy - Precious Moments of. Life means your existence as a human being. 2023 He was involved in oil, gas, precious metals and stones there. Although many people believe that co-sleeping means a child is sleeping in the same. Caroline Delbert, Popular Mechanics, 24 Aug. 2023 These states rely on water resources that are often piped in from elsewhere, making water a precious and costly resource that should not be spent to care for non-native plants like many common lawn grasses. precious through its rapid endangerment.56 Different levels of meaning. 2023 Travis Barker is spending precious time with pregnant wife Kourtney Kardashian. dotted with stellar bubbles of captured air, that wash across other moments. 2023 Feral cattle graze unusual niches all over the world, and maybe some are precious genetic outliers. Adjective The private force previously made its money by exchanging military support for cash and precious metals around the world, exploiting gold and diamond mines in places like the Central African Republic - where Russia is hoping to increase its influence. Too cold, and the wine's aging process can slow down too warm, and it can age prematurely. Storing it at the correct temperature ensures that it matures properly and maintains its flavor and aroma. Wine is sensitive to temperature fluctuations. Why is temperature important for storing wine? They can also come with features like UV-resistant doors to protect wine from harmful light. Unlike regular refrigerators, wine coolers maintain a consistent humidity level, making it ideal for wine storage. What is a wine cooler, and how does it differ from a regular refrigerator?Ī wine cooler is a specialized refrigerator designed specifically to store wine at optimal temperatures. 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Whether you're a wine connoisseur, an occasional sipper, or somewhere in between, we understand that storing your prized bottles at the optimal temperature is paramount. Elevate your wine storage with the most refined wine coolers on the market. I did have a problem connecting to their free WiFi, but the front desk clerk was unable to assist me. We had very minimal interaction with staff, who were almost invisible. The whole property was wonderfully situated, close to everything: restaurants, donuts, shopping, Walgreens, Wal Mart, etc. All in all, we love coming here, and can't wait to come back!Ī very nice, clean, modern resort, that gave us a fantastic side view of the pool, beach and ocean. We did not get the name of the bartender working the lobby bar, but she was very personable. The next evening, we saw he was playing in the lobby bar, so we stayed to have some drinks and listen to him play. Dinner at Voyagers, the upscale restaurant at Perdido, was simply fabulous, and the pianist who entertained during dinner, John Lee Sanders, is outstanding. The club level lounge offered a nice variety of foods and juices for breakfast each morning, and an open bar and appetizers during happy hour each afternoon, with wonderful views of the ocean. On this trip, we chose to stay on the top floor club level, and it was definitely worth every penny: our room was immaculately clean, large, beautifully decorated, and very comfortable the bathroom was well-stocked with fluffy towels and quality lotions and shampoos.The views of the pool area and the beach were breathtaking. Here, you have everything you need: a beautiful beach, a wonderful pool, top-notch restaurants, and top-notch entertainment. ![]() This place is great, whether you want a family vacation, or a romantic getaway. We have stayed here quite a few times, and absolutely love Perdido Beach Resort. Located directly on the Gulf of Mexico, the resort is less than two miles from the Florida state line 45 miles west of Pensacola, FL and 1 hour and 15 minutes east of Mobile, AL. Restaurants include: Latitude 30 for casual dining, Voyagers for more upscale dining and breathtaking views of the Gulf, Splash Bar & Grille poolside, Perdido Beach Resort Pizzeria and Ice Cream Shoppe, and the newest addition, Coastal Coffee, which provides grab & go items along with signature coffees. Nancy’s Lobby Lounge featuring the only piano bar on the island and much more! The resort also offers a newly renovated fitness center featuring Peloton bikes, hot tubs, a large outdoor and indoor pool, charter sailing and wave runner rentals as well as 5 restaurants located on the property. Guests enjoy incredible amenities that include private balconies, complimentary parking, complimentary WiFi, nightly entertainment in Ms. ![]() It is the only full service resort hotel in Alabama located directly on the turquoise waters of the Gulf of Mexico. Perdido Beach Resort is the premier choice for travelers visiting the beautiful Alabama Gulf Coast. Hotels near Columbia Southern University.Hotels near Big Adventure Fishing Charters.Hotels with Private Beach in Orange Beach.InterContinental (IHG) Hotels in Orange Beach. ![]() Common topics that are seen multiple times per day will be removed as long as a similar topic has been popularized recently.Any posts covering these topics outside of the megathreads will be removed. During the week we will have various megathreads covering various topics.No megathread, common or duplicate topics This does not include clips, highlights, images or videos, and official livestreams of esports competitions.Please contact us if you want to do a giveaway, we can give you further details on what we allow. ![]() No promotion of Twitch, YouTube or any other platform accounts.Posts on /r/PUBATTLEGROUNDS should ideally contain content capable of sparking discussion, and should be specifically about PUBG: BATTLEGROUNDS.This includes, but is not limited to simple screenshots of loot, your inventory, or other common PUBG sights, as well as any other contributions that would be considered low effort.No low effort posts or unrelated topics are to be posted.No gambling or linking to gambling websites. ![]()
Notably, Take-Two expects to do even better in its takings the following year, something that seems impossible without something like GTA Online built on the foundations of a brand new GTA game to propel its revenue sky high. So to achieve a goal of $8 billion, Take-Two would need to make significant acquisitions, or release nearly double the amount of games it historically has, or release at least one absolute behemoth of a game. Prior to this, Take-Two's regular annual net bookings were looking roughly in $3 billion ballpark and steadily rising - it took the acquisition of a major mobile developer to see them leap over $1 billion in the space of a single year. Notably, this record was reached thanks to the contributions of Zynga, which it acquired last year. For one, Take-Two's net bookings this past fiscal year reached $5.3 billion - a record annual high. ![]() While Take-Two doesn't explicitly name Grand Theft Auto here, it's hard to imagine any other franchise doing this work for them. We expect to sustain this momentum by delivering even higher levels of operating results in Fiscal 2026 and beyond. ![]() In Fiscal 2025, we expect to enter this new era by launching several groundbreaking titles that we believe will set new standards in our industry and enable us to achieve over $8 billion in Net Bookings and over $1 billion in Adjusted Unrestricted Operating Cash Flow. "For the last several years, we have been preparing our business to release an incredibly robust pipeline of projects that we believe will take our company to even greater levels of success. For context, fiscal 2025 will begin in April of 2024: Looking ahead, Fiscal 2025 is a highly anticipated year for our Company," the release states. In a press release issued alongside its yearly earnings report, Take-Two shared projections for the coming years, including a massive projection for fiscal years 20. Record: Capture screen activity, video and audio.Quizzing: Create and track interactive quizzes.Multicam: Combine footage from multiple devices.Hotspots: Create clickable areas in videos.Green Screen: Remove the background of your video.Enhance: Adjust colors, add a blur effect and more.Captions: Add closed captions and subtitles.Callouts: Point to and draw attention to objects.Audio Effects: Add audio effects like echo and noise reduction. ![]() Assets: Access million of royalty-free stock assets.Animation: Create motion graphics and animations.Animate: Create animated titles, transitions and more.Integrates with Google Drive, Vimeo and YouTube.Though primarily intended for e-learning creations, the application is equally adept at producing captivating family videos. Supporting a wide range of file formats, Camtasia ensures compatibility with various devices. Eschewing the complexity of high-end, costly tools, Camtasia focuses on specific media formats, templated course creation, and demos or presentations for effective learning. Why choose Camtasia?Ĭamtasia Studio has quickly gained popularity as an e-authoring and learning tool for Windows PCs. Post-recording, users can effortlessly rearrange visual elements to showcase particular lesson segments. ![]() Its intuitive navigation facilitates zooming into specific screen sections, panning across areas, and creating animations for videos. Instead, its basic editing functionalities emphasize instructional aspects, positioning Camtasia as a robust e-authoring app. Editing capabilities: An overviewĪlthough equipped with editing tools, Camtasia doesn't rival comprehensive applications like Adobe Premiere. Even if video editing isn't your forte, you can record slideshows, transform them into animations, and share them with your network. Unlike competing free tools, Camtasia's zooming prowess avoids pixelation, delivering crisp visuals.Ĭamtasia boasts a myriad of features to amplify your visuals, such as adjustable cursor opacity, highlight effects, and layered images. This functionality paves the way for bite-sized demos of specific on-screen segments. The software enables users to capture selected areas or entire windows. Effortlessly merge video files or images, record cursor activity, and zoom in on details for a visually striking end product.īeyond basic video editing, Camtasia shines in screen recording. The software shines in creative content projects, software demos, and e-learning presentations, to name a few. 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While predominantly preferred by Windows users, Mac aficionados can also revel in its capabilities. In no time, Camtasia Studio has emerged as a trustworthy video editing ally. A plethora of editing features, an intuitive design, and much more Notably, the software seamlessly integrates audio tracks and video files, streamlining your creative endeavors. The application empowers creators to produce eye-catching tutorials, guides, slideshows, and presentations, among other multimedia content. Professional video capture software that records virtually any activity on your monitor including gaming and screen activity with a great video editor.Ĭamtasia reigns as a premier video editing software for Windows enthusiasts, offering a plethora of features to craft, refine, and disseminate videos across the digital sphere. |
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